Dihydrocoumarin, kakara, whakamahia i roto i te kai, whakamahia hoki hei whakakapinga coumarin, whakamahia rite hā Paku; Whakaranuhia te kirīmi, te kokonati, te hā hinamona; Ka whakamahia ano hei kakara tupeka.
He paitini te dihydrocoumarin
Ko te Dihydrocoumarin ehara i te mea paitini. Ko te Dihydrocoumarin he hua taiao e kitea ana i roto i nga rhinoceros vanilla kowhai. Ka mahia e te hauwai o te coumarin i te aroaro o te whakakorikori nickel i te 160-200 ℃ me te pehanga. Ka taea hoki te whakamahi hei rauemi mata, ka whakamarohia i roto i te wairewa wai kawakore ki te whakaputa i te waikawa o-hydroxyphenylpropionic, te matewai, te kati-koropiko kua whiwhi.
Te ahua rokiroki
Ka kati me te pouri, ka rongoa ki te waahi makariri me te maroke, ko te waahi i roto i te putea he iti rawa i raro i nga whakaaetanga haumaru, ka whakakiia ki te whakamarumaru hauota. Rokiroki i roto i te whare putunga hauhautanga, whai hau. Matara atu i te ahi, te wai. Me penapena motuhake mai i te oxidizer, kaua e uru ki te rokiroki. Kua rite ki te momo me te nui o nga taputapu ahi.
Te ako in vitro
In vitro enzymatic assay, dihydrocoumarin induced concentration-dependent inhibition of SIRT1 (IC50 of 208μM). Ko nga whakahekenga o te mahi deacetylase SIRT1 i kitea ahakoa i nga potae micromolar (85±5.8 me te 73±13.7% mahi i te 1.6μM me te 8μM, ia). I whakakorehia ano te Microtubule SIRT2 deacetylase i roto i te ahua o te horopeta-whakaaro (IC50 o 295μM).
I muri i nga haora 24 o te whakakitenga, ka piki ake te cytotoxicity te dihydrocoumarin (1-5mM) i roto i nga raina pūtau TK6 i runga i te tikanga e whakawhirinaki ana ki te horopeta. Ko te Dihydrocoumarin (1-5mM) te whakanui ake i te apoptosis i roto i nga raina pūtau TK6 i runga i te tikanga e whakawhirinaki ana ki te horopeta i te wa 6-haora. Ko te horopeta 5mM o te dihydrocoumarin te whakanui ake i te apoptosis i te wa 6 haora i roto i te raina pūtau TK6. I muri i te 24-haora o te whakaaturanga, ka piki te dihydrocoumarin (1-5mM) i te p53 lysine 373 me te 382 acetylation i roto i te tikanga horopeta-ti'aturi i roto i te raina pūtau TK6.
Wā tuku: Nov-01-2024